UPLC/FLR/QTof MS Analysis of Procainamide-Labeled N-Glycans
نویسندگان
چکیده
UltraPerformance LC (UPLC) in hydrophilic interaction chromatography (HILIC) separation mode is becoming a routine and widely recognized technique for rapid, efficient, sensitive, and reproducible analysis of 2-aminobenzamide (2-AB) labeled glycans. Even though 2-AB is now the most common glycan labeling reagent, its use is limited by low MS sensitivity. Recent reports on LC/FLR/MS of glycans tagged with procaimanide demonstrate this alternative reagent would be advantageous for improving MS ionization efficiency without compromising LC separation.
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Rapid Preparation of Released N-Glycans for HILIC Analysis Using a Novel Fluorescence and MS-Active Labeling Reagent
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